Amitani, I., Baskin, R.J., and Kowalczykowski, S.C. (2006).
Visualization of Rad54, a chromatin-remodeling protein, translocating on single DNA molecules.
Mol. Cell, 23, 143-148.  [Abstract]  [Full Text]  [PDF]  [Supplemental Data]  [Upstream Translocation Movie]  [Downstream Translocation Movie]

Spies, M. and Kowalczykowski, S.C. (2006).
The RecA-binding locus of RecBCD enzyme defines a generalized domain for recruitment of DNA strand exchange proteins.
Mol. Cell, 21, 573-580.  [Abstract]  [Full Text]  [PDF] [Supplemental Data]

Handa, N., Bianco, P.R., Baskin, R.J., and Kowalczykowski, S.C. (2005).
Direct visualization of RecBCD movement reveals co-translocation of the RecD motor after c-recognition.
Mol. Cell, 17, 745-750.  [Abstract]  [Full Text]  [PDF]  [Supplemental Data]  [Movie 1 - Chi⁰]  [Movie 2 - Chi⁺]

Singleton, M. R., Dillingham, M.S., Gaudier, M., Kowalczykowski, S.C., and Wigley, D. B. (2004).
Crystal structure of RecBCD reveals a machine for processing DNA breaks.
Nature, 432, 187-193.   [Abstract]  [Full Text]  [PDF]
(see News & Views  [Full Text]  [PDF])
(see Nature "Highlights"  [Full Text])

Mazina, O.M., Mazin, A.V., Nakagawa T., Kolodner, R.D., and Kowalczykowski, S.C. (2004). 
Saccharomyces cerevisiae Mer3 helicase stimulates 3’® 5’ DNA heteroduplex extension by Rad51: Implications for crossover control in meiotic recombination.
Cell, 117, 47-56  [Abstract]  [Full Text]  [PDF]
(see comments in Review  [Abstract]  [Full Text]  [PDF])
(see News & Information at UC Davis News Service  [Full Text]  [Printable Version])

Spies, M., Bianco, P.R., Dillingham, M.S., Handa, N., Baskin, R.J., and Kowalczykowski, S.C.  (2003).
A molecular throttle: The recombination hotspot c controls DNA translocation by the RecBCD helicase.
Cell, 114, 647-654.  [Abstract]  [Full Text]  [PDF]  [Featured Article]
(see News & Features at Bio.com  [Full Text]  [Printable Version])
(see News & Information at UC Davis News Service  [Full Text]  [Printable Version])
(see News & Information at UC Davis News Service  [Full Text]  [Printable Version])

Morimatsu, K. and Kowalczykowski, S.C.  (2003).  
RecFOR proteins load RecA protein onto gapped DNA to accelerate DNA strand exchange: A universal step of recombinational repair. 
Mol. Cell, 11, 1337-1347  [Abstract]  [Full Text]  [PDF]

Veaute, X., Jeusset, J., Soustelle, C., Kowalczykowski, S.C. , Le Cam, E., and Fabre, F. (2003). 
The Srs2 helicase prevents homologous recombination by disrupting Rad51 nucleofilaments. 
Nature, 423, 309-312  [Abstract]  [Full Text]  [PDF]
(see News & Views  [Full Text]  [PDF])

Dillingham, M.S., Spies, M., and Kowalczykowski, S.C. (2003). 
RecBCD enzyme is a bipolar DNA helicase.  
Nature, 423, 893 - 897  [Abstract]  [Full Text]  [PDF]
(see News & Views in Nature Structural Biology 10, 499 - 500 (2003)  [Full Text]  [PDF])
(see Highlight in Nature Reviews Molecular Cell Biology 4, 513 (2003); doi:10.1038/nrm1162 [Full Text]  [PDF])
(Must Read paper by Faculty of 1000 [Full Text])
(see News & Information at UC Davis News Service  [Full Text]  [Printable Version])

Alexeev, A., Mazin, A.V., and Kowalczykowski, S.C. (2003).
Rad54 protein possesses chromatin remodeling activity stimulated by the Rad51-ssDNA nucleoprotein filament.
Nature Struct. Biol., 10, 182-186 (published 10 February 2003 as doi.10.1038/nsb901).  [Abstract]  [Full Text]  [PDF] [Online cover]
(see Highlight in Nature Reviews Molecular Cell Biology 4, 261 (2003); doi:10.1038/nrm1089   [Full Text]  [PDF])
(twice Recommended paper by Faculty of 1000 [Full Text]))
(see News & Information at UC Davis News Service  [Full Text]  [Printable Version])

Kantake, N., Madiraju, M.V.V.M., Sugiyama, T., and Kowalczykowski, S.C. (2002).
Annealing of ssDNA complexed with cognate ssDNA-binding protein by E. coli RecO protein: A common step in genetic recombination. 
Proc. Natl. Acad. Sci. USA, 99, 15327-15332 (published November 18, 2002, as 10.1073/pnas.252633399).  [Abstract]  [Full Text]  [PDF]
(Recommended paper by Faculty of 1000 [Full Text])

Kowalczykowski, S.C. (2002).
Molecular mimicry connects BRCA2 to Rad51 and recombinational DNA repair. 
Nature Struct. Biol., 9, 897-899.  [Abstract]  [Full Text]  [PDF]

Bianco, P.R., Brewer, L.R., Corzett, M., Balhorn, R., Yeh, Y., Kowalczykowski, S.C., and Baskin, R.J. (2001). 
Processive translocation and DNA unwinding by individual RecBCD enzyme molecules. 
Nature, 409, 374-378.  [Abstract]  [Full Text]  [PDF] [Supplementary Information]
Movies!   37°C:  1 mM ATP        23°C:   1 mM ATP;    250 µM ATP #1;    250 µM ATP #2;    no ATP
(see coverage in Biophotonics (2001) 8, 48-49   [TOC]  [PDF])

Press coverage:
Capitol Public Radio - January 17, 2001 - Full transcript
San Francisco Chronicle - January 20, 2001 - excerpt   
Full article - "Watch DNA Unzip in Micromovie-Molecular action film highlights potential of nanotechnology"   Printer-friendly version
San Francisco Chronicle - January 24, 2001 - excerpt   
Full article: "A Little Spark Of Inspiration"   Printer-friendly version
San Francisco Chronicle - January 26, 2001 - excerpt   
Full article: "Never Metatag We Didn't Like"   Printer-friendly version
News & Information at UC Davis News Service  [Full Text]  [Printable Version])
Nanotech Dreams - The Scientist 16[5]:34, Mar. 4, 2002 - Excerpt Full article
Davis Community Television - February 8, 2001 7:00 PM  - "Get The Word Out." Double helix unwinds on camera. Stephen Kowalczykowski interviewed by Jon Price. (Rerun on Tues Feb 13, 2001 at 7:30 PM). [VIDEO]

Kowalczykowski, S.C.  (2000). 
Some assembly required….  
Nature Structural Biology, 7, 1087-1089.  [Abstract]  [Full Text]  [PDF]

Mazin, A.V., Bornarth, C.J., Solinger, J.A., Heyer, W.-D., and Kowalczykowski, S.C. (2000). 
Rad54 Protein is targeted to pairing loci by the Rad51 nucleoprotein filament. 
Mol. Cell, 6, 583–592. [Abstract]  [Full Text]  [PDF]

Bianco, P.R. and Kowalczykowski, S.C.  (2000). 
The translocation step size and mechanism of the RecBC helicase.  
Nature, 405, 368-372.  [Abstract]  [Full Text]  [PDF]
(see Dispatch in Current Biology: [Abstract]  [Full Text]  [PDF])
(see News & Information at UC Davis News Service  [Full Text]  [Printable Version])

Zaitsev, E.N. and Kowalczykowski, S.C. (2000). 
A novel pairing process promoted by Escherichia coli RecA protein: Inverse DNA and RNA strand exchange. 
Genes & Dev. 14, 740-749.  [Abstract]  [Full Text]  [PDF]

Arnold, D.A. and Kowalczykowski, S.C. (2000). 
Facilitated loading of RecA protein is essential to recombination by RecBCD enzyme.
J. Biol. Chem., 275, 12261-12265.  [Abstract]  [Full Text]  [PDF]

Mazin, A.V., Zaitseva, E., Sung, P., and Kowalczykowski, S.C. (2000).
Tailed duplex DNA is the preferred substrate for Rad51 protein-mediated homologous pairing. 
EMBO J., 19, 1148-1156.  [Abstract]  [Full Text]  [PDF]

Mazin, A.V. and Kowalczykowski, S.C. (1999).
A novel property of the RecA nucleoprotein filament: activation of dsDNA for strand exchange in trans.
Genes & Dev., 13, 2005-2016.  [Abstract]  [Full Text]  [PDF]

Harmon, F.G., DiGate, R.J., and Kowalczykowski, S.C. (1999).
RecQ helicase and topoisomerase III comprise a novel DNA strand passage function: a conserved mechanism for control of DNA recombination.
Mol. Cell, 3, 611-620.  [Abstract]  [Full Text]  [PDF]
(see Dispatch in Current Biology: [Abstract]  [Full Text]  [PDF])

Churchill, J.J., Anderson, D.G., and Kowalczykowski, S.C. (1999).
The RecBC enzyme loads RecA protein onto ssDNA asymmetrically and independently of c, resulting in constitutive recombination activation.
Genes & Dev. 13, 901-911. [Abstract]  [Full Text]  [PDF]

Anderson, D.G. and Kowalczykowski, S.C. (1998).
Reconstitution of an SOS response pathway: derepression of transcription in response to DNA breaks.
Cell, 95, 975-979.  [Abstract]  [Full Text]  [PDF]

Sugiyama, T., New, J.H., and Kowalczykowski, S.C. (1998).
DNA Annealing by Rad52 protein is stimulated by specific interaction with the complex of replication protein A and single-stranded DNA. 
Proc. Natl. Acad. Sci. USA, 95, 6049-6054.   [Abstract]   [Full Text]  [PDF]

Seitz, E.M., Brockman, J.P., Sandler, S.J., Clark, A.J., and Kowalczykowski, S.C. (1998).
RadA protein is an archaeal RecA protein homolog that catalyzes DNA strand exchange. 
Genes & Dev., 12, 1248-1253.   [Abstract]   [Full Text]  [PDF]  
[Genes & Dev. cover] [Genes & Dev. cover.jpeg]

Mazin, A.V. and Kowalczykowski, S.C. (1998).
The function of the secondary DNA-binding site of RecA protein during DNA strand exchange.
EMBO J., 17, 1161-1168.   [Abstract]   [Full Text]  [PDF]
(see review in CHEMTRACTS-Biochemistry and Molecular Biology, 11, 777-784, 1998)

New, J.H., Sugiyama, T., Zaitseva, E., and Kowalczykowski, S.C. (1998).
Rad52 protein stimulates DNA strand exchange by Rad51 and replication protein A. 
Nature, 391, 407-410.   [Abstract]   [Full Text]  [PDF]  
(see Nature News & Views: [News&Views] [N&V PDF])

Tracy, R.B., Chédin, F., and Kowalczykowski, S.C. (1997).
The recombination hot spot Chi is embedded within islands of preferred DNA pairing sequences in the E. coli genome. 
Cell, 90, 205-206.   [Abstract]  [Full Text]  [PDF]

Some classics:                                  (top)

Anderson, D.G. and Kowalczykowski, S.C. (1997).
The translocating RecBCD enzyme stimulates recombination by directing RecA protein onto ssDNA in a c-regulated manner. 
Cell, 90, 77-86.   [Abstract]  [Full Text]  [PDF]
(see Dispatch in Current Biology: [Abstract]  [Full Text]  [PDF])

Anderson, D.G. and Kowalczykowski, S.C. (1997).
The recombination hot spot c is a regulatory element that switches the polarity of DNA degradation by the RecBCD enzyme. 
Genes & Dev., 11, 571-581.   [Abstract]  [PDF]

Tracy, R.B. and Kowalczykowski, S.C. (1996).
In vitro selection of preferred DNA pairing sequences by the Escherichia coli RecA protein.
Genes & Dev., 10, 1890-1903.   [Abstract]  [PDF]

Dixon, D.A. and Kowalczykowski, S.C. (1991).
Homologous pairing in vitro stimulated by the recombination hotspot, Chi.
Cell, 66 361-371.   [Abstract]   [PDF]

Menetski, J.P., Bear, D.G., and Kowalczykowski, S.C. (1990).
Stable DNA heteroduplex formation by the Escherichia coli recA protein in the absence of ATP hydrolysis. 
Proc. Natl. Acad. Sci. U.S.A. 87, 21-25.   [Abstract]  [PDF]

Kowalczykowski, S.C. and Krupp, R.A. (1987).
Effects of the Escherichia coli SSB protein on the single-stranded DNA-dependent ATPase activity of Escherichia coli RecA protein: Evidence that SSB protein facilitates the binding of RecA protein to regions of secondary structure within single-stranded DNA.
J. Mol. Biol. 193, 97-113.   [Abstract]  [PDF]