Amitani, Ichiro

 

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Ichiro Amitani

B.S., Physics, 1991 - Kanazawa University, Japan

M.S., Physics, 1993 - Kanazawa University, Japan

Ph.D., Life Science, 2000 - Kanazawa University, Japan

Postdoctoral Scholar, Biophysics,  2002-2008, University of California, Davis

Assistant Project Scientist, 2008-present, University of California, Davis

   

Sakamoto, T., Amitani, I., Yokota, E., and Ando, T. (2000).
Direct observation of processive movement by individual myosin V molecules.
Biochem. Biophys. Res. Comm., 272
, 586-590. PMID: 10833456.
 

Amitani, I., Sakamoto, T., and Ando, T. (2001).
Link between the enzymatic kinetics and the mechanical behavior in an actomyosin motor.
Biophys. J., 80
, 379-397. PMID: 11159410; PMCID: PMC1301241.

Amitani, I., Baskin, R.J., and Kowalczykowski, S.C. (2006)
Visualization of Rad54, a chromatin-remodeling protein, translocating on single DNA molecules.
Mol. Cell,
23, 143-148PMID: 16818238. [Abstract] [Full Text] [PDF] [Supplemental Data] [Upstream Translocation Movie] [Downstream Translocation Movie]
(Third most requested article in August 2006 in Molecular Cell [PDF])

Galletto, R., Amitani, I., Baskin, R.J., and Kowalczykowski, S.C. (2006)
Direct observation of individual RecA filaments assembling on single DNA molecules.
Nature,
443, 875-878. PMID: 16988658.
[Abstract] [Full Text] [PDF] [Supplementary Information]
(see
ACS Chemical Biology Spotlight Filaments under the flashlight [PDF])
(see Bio.com Watching DNA repair in real time 
[PDF])
(see EMBO meeting report: How to exchange your partner [PDF])
(see
MedicalNewsToday.com Direct observations of DNA are giving new insights into  how genetic material is copies and repaired [PDF])
(see
News-Medical.net  Direct observations of DNA provides new insights into how genetic material is copied and repaired [PDF])
(see
PhysOrg.com  Watching DNA repair in real time [PDF])
(see
nnseek.com  Watching DNA repair in real time [PDF])
(see
ScienceDaily.com  Watching DNA repair in real time [PDF])
(see Structure Preview: RecA Assembly, One Molecule at a Time  [PDF])
(
see
UC Davis News and Information Watching DNA repair in real time [PDF] [HTML])

Nimonkar, A., Amitani, I., Baskin, R.J., and Kowalczykowski, S.C. (2007)
Single-molecule imaging of Tid1/Rdh54, a Rad54 homolog that translocates on duplex DNA and can disrupt joint molecules.
J. Biol. Chem, 282
, 30776-30784
PMID: 17704061. [Abstract] [Full Text] [PDF] [Supplemental Data] [Movie 1] [Movie 2] [Movie 3] [Movie 4]

Spies, M., Amitani, I., Baskin, R.J., and Kowalczykowski, S.C. (2007)
RecBCD enzyme switches lead motor subunits in response to
c-recognition.
Cell, 131, 16, 694-705 (doi:10.1016/j.cell.2007.9.023). PMID: 18022364. PMCID: PMC2151923. 
[Abstract] [Full Text] [PDF] [Supplemental Data]
(see Cell  "RecBCD: The Supercar of DNA Repair
[PDF])
(see Egghead Blog about research at UC Davis: A DNA machine running on two engines [PDF])

Hilario, J., Amitani, I., Baskin, R.J., and Kowalczykowski, S.C. (2009)
Direct imaging of human Rad51 nucleoprotein dynamics on individual DNA molecules.

Proc. Natl. Acad. Sci. U.S.A., 106
, 361-368 (published online January 2, 2009, doi: 10.1073/pnas.0811965106.) PMID: 19122145; PMCID: PMC2613362
[Abstract] [Full Text] [PDF] [Supporting Information] [Movie 1] [Movie 2] [Movie 3]
(see About Medicine: Fluorescent microscopy captures the repair protein Rad51 as it assembles into a filament on DNA [PDF])
(see Biocompare News: Human DNA repair process recorded in action [PDF])
(see Biology News Net: Human DNA repair process recorded in action [PDF])
(see Biophotonics: Mending broken DNA [PDF])

(see e! Science News: Human DNA repair process recorded in action [PDF])
(see EurekAlert: Human DNA repair process recorded in action [PDF])
(see Insciences Organisation: Human DNA repair process recorded in action [PDF])

(see Machines Like Us, News: Human DNA repair process recorded in action [PDF])
(see MacNewsWorld: EurekAlert: Human DNA repair process recorded in action [PDF])
(see Medical News Today: Fluorescent microscopy captures the repair protein Rad51 as it assembles into a filament on DNA [PDF])
(see Newsguide.us: Human DNA repair process recorded in action [PDF])
(see Newsweek, National News: Human DNA repair process recorded in action (Video) [PDF])
(see Overwhelming Evidence: Human DNA repair process video - by chance? [PDF])
(see Phys.org/News: Human DNA repair process recorded in action (Video) [PDF])
(see ScienceBlog: Human DNA repair process recorded in action [PDF])
(see Science Codex: Human DNA repair process recorded in action [PDF])
(see Science Daily: Human DNA repair process recorded in action [PDF])
(see ScienceMode.com: Human DNA repair process recorded in action [PDF])
(see The Sensuous Curmudgeon: Human DNA repair process recorded [PDF])
(see UCDavis News and Information: Human DNA repair process recorded in action Caught in the act: Our DNA under repair  [Video] [Printable])
(see Uncommon Descent: Human DNA repair process video - by chance? [PDF])

Carreira, A., Hilario, J., Amitani, I., Baskin, R.J., Shivji, M.K.K., Venkitaraman, A.R., and Kowalczykowski, S.C. (2009)
The BRC repeats of BRCA2 modulate the DNA-binding selectivity of RAD51.

Cell, 136,
1032-1043, (doi:10.1016/j.cell.2009.02.019). PMID: 19303847; PMCID: PMC2669112  [Abstract] [Full Text] [PDF] [Supplemental Data]
(see Cell, In this Issue: BRCA2 Focuses RAD51 for Repair  [PDF])
Access the recommendation on F1000Prime

Handa, N., Amitani, I., Gumlaw, N., Sandler, S.J., and Kowalczykowski, S.C. (2009)
Single molecule analysis of a red fluorescent RecA Protein reveals a defect in nucleoprotein filament nucleation that relates to its reduced biological functions.
J. Biol. Chem.,
284, 18664-73 (published online May 5, 2009, doi:10.1074/jbc.M109.004895). PMID: 19419960; PMCID: PMC2707236 
[Abstract] [Full Text] [PDF]

Amitani, I., Liu, B., Dombrowski, C.C., Baskin, R.J., and Kowalczykowski, S.C. (2010)
Watching individual proteins acting on single molecules of DNA.
In:
Single Molecule Tools: Fluorescence Based Approaches, Part A, Methods Enzymol., 472, Nils G. Walters, editor, Burlington: Academic Press, 261-291
ISBN: 978-0-12-374954-3; doi: 10.1016/S0076-6879(10)72007-3PMID: 20580968; PMCID: PMC3119597  [Abstract] [Full Text] [PDF]

Forget, A.L., Dombrowski, C.C., Amitani, I., and Kowalczykowski, S.C. (2013)
Exploring protein-DNA interactions in 3D using in situ construction, manipulation, and visualization of individual DNA dumbbells with optical traps, microfluidics, and fluorescence microscopy.
Nature Protocols, 8
, 525-538. 
PMID: 23411634; PMCID: PMC4329291. [Abstract] [Full Text] [PDF] [Supplementary Figure 1]