Amitani, Ichiro

 

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Ichiro Amitani

B.S., Physics, 1991 - Kanazawa University, Japan

M.S., Physics, 1993 - Kanazawa University, Japan

Ph.D., Life Science, 2000 - Kanazawa University, Japan

Postdoc., Biophysics,  2002-pres., University of California, Davis

 

   

Sakamoto, T., Amitani, I., Yokota, E., and Ando, T. (2000).
Direct observation of processive movement by individual myosin V molecules.
Biochem. and Biophys. Res. Comm., 272
,
586-590. PMID: 10833456.
 

Amitani, I., Sakamoto, T., and Ando, T. (2001).
Link between the enzymatic kinetics and the mechanical behavior in an actomyosin motor.
Biophys. J., 80
, 379-397. PMID: 11159410; PMCID: PMC1301241.

Amitani, I., Baskin, R.J., and Kowalczykowski, S.C. (2006).
Visualization of Rad54, a chromatin-remodeling protein, translocating on single DNA molecules.
Mol. Cell,
23, 143-148PMID: 16818238. [Abstract] [Full Text] [PDF] [Supplemental Data] [Upstream Translocation Movie] [Downstream Translocation Movie]
(Third most requested article in August 2006 in Molecular Cell [PDF])

Galletto, R., Amitani, I., Baskin, R.J., and Kowalczykowski, S.C. (2006).
Direct observation of individual RecA filaments assembling on single DNA molecules.
Nature,
443, 875-878. PMID: 16988658.
 [Abstract] [Full Text] [PDF]  [Supplemental Data]
    (see
ACS Chemical Biology Spotlight  [PDF])
    (see Bio.com  
[PDF])
    (see
MedicalNewsToday.com  [PDF])
    (see
News-Medical.net  [PDF])
    (see
PhysOrg.com  [PDF])
    (see
nnseek.com  [PDF])
    (see
ScienceDaily.com  [PDF])
    (see
UC Davis News and Information  [PDF] [HTML]

Nimonkar, A., Amitani, I., Baskin, R.J., and Kowalczykowski, S.C. (2007).
Single-molecule imaging of Tid1/Rdh54, a Rad54 homolog that translocates on duplex DNA and can disrupt joint molecules.
J. Biol. Chem, 282
, 30776-30784
PMID: 17704061. [Abstract] [Full Text] [PDF] [Supplementary Information]

Spies, M., Amitani, I., Baskin, R.J., and Kowalczykowski, S.C. (2007).
RecBCD enzyme switches lead motor subunits in response to
c-recognition.
Cell, 131, 16, 694-705 (doi:10.1016/j.cell.2007.9.023). PMID: 18022364. PMCID: PMC2151923. 
[Abstract]  [Full Text]  [PDF]  [Supplementary Information]
(see Cell  "RecBCD: The Supercar of DNA Repair" 
[PDF])

Hilario, J., Amitani, I., Baskin, R.J., and Kowalczykowski, S.C. (2009).
Direct imaging of human Rad51 nucleoprotein dynamics on individual DNA molecules.

Proc. Natl. Acad. Sci. U.S.A., 106
, 361-368 (published online January 2, 2009, doi: 10.1073/pnas.0811965106. PMID: 19122145; PMCID: PMC2613362
[Abstract]  [Full Text]  [PDF]

Carreira, A., Hilario, J., Amitani, I., Baskin, R.J., Shivji, M.K.K., Venkitaraman, A.R., and Kowalczykowski, S.C. (2009).
The BRC repeats of BRCA2 modulate the DNA-binding selectivity of RAD51.

Cell, 136,
1032-1043, (doi:10.1016/j.cell.2009.02.019). PMID: 19303847; PMCID: PMC2669112  [Abstract]  [Full Text]  [PDF] [Supplemental Data]

Handa, N., Amitani, I., Gumlaw, N., Sandler, S.J., and Kowalczykowski, S.C. (2009).
Single molecule analysis of a red fluorescent RecA Protein reveals a defect in nucleoprotein filament nucleation that relates to its reduced biological functions.
J. Biol. Chem.,
284, 18664-73 (published online May 5, 2009, doi:10.1074/jbc.M109.004895). PMID: 19419960; PMCID: PMC2707236 
[Abstract]  [Full Text]  [PDF]

Amitani, I., Liu, B., Dombrowski, C.C., Baskin, R.J., and Kowalczykowski, S.C. (2010).
Watching individual proteins acting on single molecules of DNA.
In:
Single Molecule Tools: Fluorescence Based Approaches, Part A, Methods Enzymol., 472, Nils G. Walters, editor, Burlington: Academic Press, 261-291
ISBN: 978-0-12-374954-3; doi: 10.1016/S0076-6879(10)72007-3PMID: 20580968; PMCID: PMC3119597  [Abstract] [Full Text] [PDF]

Forget, A.L., Dombrowski, C.C., Amitani, I., and Kowalczykowski, S.C. (2013).
Exploring protein-DNA interactions in 3D using in situ construction, manipulation, and visualization of individual DNA dumbbells with optical traps, microfluidics, and fluorescence microscopy.
Nature Protocols, 8
, 525-538. 
PMID: 23411634 [Abstract]  [Full Text]  [PDF]