Galletto, Roberto

 

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Roberto Galletto

B.S., 1996, Biology - University of Genoa, Genoa, Italy

Ph.D., 2002, Biochemistry - University of Texas Medical Branch, Galveston, TX

Postdoctoral Fellow, 2003-2004, Biochemistry - University of Texas Medical Branch, Galveston, TX

Postdoctoral Fellow, 2004-2007 - University of California, Davis, CA

Jeane B. Kempner Postdoctoral Fellowship, 2003-2005

  Current address:

Assistant Professor
Washington University
Biochemistry & Molecular Biophysics
Campus Box 8231
One Brookings Drive
St. Louis, MO 63130
Tel: (314) 362-4368
galletto@wustl.edu

Galletto, R., Attanasio, F., Dossi, E., Schulein, M., and Rialdi, G. (1998).
Heat of protonation of endoglucanase V catalytic domain (EGV-core) from Humicola insolens.
Thermochimica Acta, 321,
17-21.

Attanasio, F., Bruschi, M., Candiano, G., Galletto, R., Musante, L., Schulein, M., and Rialdi, G. (1999).
Analytical titration curves of glycosyl hydrolase Cel45 by combined isolectric focusing-electrophoresis.
Electrophoresis, 20,
1403-1411.

Galletto, R., Rajendran, S, and Bujalowski, W. (2000).
Interactions of nucleotide cofactors with the Escherichia coli replication factor DnaC protein.
Biochemistry, 39,
12959-12969.

Jezewska, M.J., Rajendran, S., Galletto, R., and Bujalowski, W.  (2001).
Kinetic mechanisms of rat polymerase b-ssDNA interactions.  Quantitatiave fluorescence stopped-flow analysis of the formation of the (Pol b)16 and (Pol b)5 ssDNA binding modes.
J. Mol. Biol., 313,
977-1002.

Jezewska, M.J., Galletto,R., and Bujalowski, W. (2002).
Dynamics of gapped DNA recognition by human polymerase b.
J.
Biol. Chem., 277, 20316-20327.

Galletto, R., and Bujalowski, W. (2002).
Kinetics of the E. coli replication factor DnaC protein-nucleotide interactions. II. Fluorescence anisotropy and transient, dynamic quenching stopped-flow studies of the reaction intermediates.
Biochemistry, 41,
8921-8934.

Galletto, R., and Bujalowski, W. (2002).
The E. coli replication factor DnaC protein exists in two conformations with different nucleotide binding capabilities. I. Determination of the binding mechanism using ATP and ADP fluorescent analogs.
Biochemistry, 41,
8907-8920.

Jezewska, M.J., Galletto,R., and Bujalowski, W. (2003).
Rat polymerase b gapped DNA interactions. Antagonistic effects of the 5 terminal PO4- group and magnesium on the enzyme binding to the gapped DNA with different ssDNA gaps.
Cell Biochem. and Biophys., 38,
125-160.

Jezewska, M.J., Galletto R., Bujalowski, W. (2003).
Rat polymerase β binds double-stranded DNA using exclusively the 8-kDa domain. Stoichiometries, intrinsic affinities, and cooperativities.
Biochemistry, 42,
5955-5970.

Galletto R., Jezewska, M.J., and Bujalowski, W. (2003).
Interactions of the Escherichia coli DnaB helicase hexamer with the replication factor the DnaC protein. Effect of nucleotide cofactors and the ssDNA on protein-protein interactions and the topology of the complex.
J. Mol. Biol., 329,
441-465.

Jezewska, M.J., Galletto, R., and Bujalowski, W. (2003).
Tertiary conformation of the template-primer and gapped DNA substrates in complexes with rat polymerase beta. Fluorescence energy transfer studies using the multiple donor-acceptor approach.
Biochemistry, 42,
11864-78.

Galletto R., Maillard, R., Jezewska, M.J., and Bujalowski, W. (2004).
Global conformation of the Escherichia coli replication factor DnaC protein in absence and presence of nucleotide cofactors.
Biochemistry, 43,
10988-1001.

 Galletto R., Jezewska, M.J., and Bujalowski, W. (2004).
Multistep sequential mechanism of Escherichia coli helicase PriA protein-ssDNA interactions. Kinetics and energetics of the active ssDNA-searching site of the enzyme.
Biochemistry, 43,
11002-11016.

Galletto R., Jezewska, M.J., and Bujalowski, W. (2004).
Unzipping mechanism of the double-stranded DNA unwinding by a hexameric helicase: quantitative analysis of the rate of the dsDNA unwinding, processivity and kinetic step-size of the Escherichia coli DnaB helicase using rapid quench-flow method.
J. Mol. Biol., 343,
83-99.

Galletto R., Jezewska, M.J., and Bujalowski, W. (2004).
Unzipping mechanism of the double-stranded DNA unwinding by a hexameric helicase: the effect of the 3' arm and the stability of the dsDNA on the unwinding activity of the Escherichia coli DnaB helicase.
J. Mol. Biol., 343,
101-114.

Jezewska, M.J., Galletto R., and Bujalowski, W. (2004).
Interactions of the RepA helicase hexamer of plasmid RSF1010 with the ssDNA. Quantitative analysis of stoichiometries, intrinsic affinities, cooperativities, and heterogeneity of the total ssDNA-binding site.
J. Mol. Biol., 343,
115-136.

Galletto, R., Jezewska, M.J., Maillard, R., and Bujalowski, W. (2005).
Kinetics of allosteric conformational transition of a macromolecule prior to ligand binding.  Analysis of stopped-flow kinetic experiments.
Cell Biochem. and Biophys., 42, 121-44.

Galletto, R., Jezewska, M.J., Maillard, R., and Bujalowski, W. (2005).
The nucleotide-binding site of the Escherichia coli DnaC protein.  Molecular topography of DnaC protein - nucleotide cofactor complexes.

Cell Biochem. and Biophys., 43,
331-354.

Galletto, R., Jezewska, M.J., and Bujalowski, W. (2005).
Kinetic mechanism of rat polymerase b - dsDNA interactions.  Fluorescence stopped-flow analysis of the cooperative ligand binding to a two-site one-dimensional lattice.
Biochemistry, 44,
1251-67.

Galletto, R., Amitani, I., Baskin, R.J., and Kowalczykowski, S.C. (2006)
Direct observation of individual RecA filaments assembling on single DNA molecules.
Nature,
443, 875-878. PMID: 16988658.
[Abstract] [Full Text] [PDF] [Supplementary Information]
(see
ACS Chemical Biology Spotlight Filaments under the flashlight [PDF])
(see Bio.com Watching DNA repair in real time 
[PDF])
(see EMBO meeting report: How to exchange your partner [PDF])
(see
MedicalNewsToday.com Direct observations of DNA are giving new insights into  how genetic material is copies and repaired [PDF])
(see
News-Medical.net  Direct observations of DNA provides new insights into how genetic material is copied and repaired [PDF])
(see
PhysOrg.com  Watching DNA repair in real time [PDF])
(see
nnseek.com  Watching DNA repair in real time [PDF])
(see
ScienceDaily.com  Watching DNA repair in real time [PDF])
(see Structure Preview: RecA Assembly, One Molecule at a Time  [PDF])
(
see
UC Davis News and Information Watching DNA repair in real time [PDF] [HTML])

Galletto, R. and Kowalczykowski, S.C. (2007).
RecA.
Curr. Biol, 17
, R395-R397. PMID: 17550759.
[Full Text] [PDF]