Sugiyama, Tomohiko



Tomo Sugiyama.jpg (35941 bytes) Tomohiko Sugiyama

B.S., Biology, 1987 - Osaka University, Osaka, Japan

M.S., Physiology, 1989 - Osaka University, Osaka, Japan

Ph.D., Physiology, 1992 - Osaka University, Osaka, Japan

Postdoctoral Fellow, 1995-2003 - University of California, Davis, CA

  Current address:

Associate Professor
Department of Biological Sciences
Ohio University
Life Science Research Facility - Rm 223
Athens, OH 45701
Tel: (740) 597-1927

Sugiyama, T., Zaitseva, E.M., and Kowalczykowski, S.C. (1997).
A single-stranded DNA-binding protein is needed for efficient presynaptic complex formation by the Saccharomyces cerevisiae Rad51 protein.
J. Biol. Chem., 272, 7940-7945. PMID: 9065463. [Abstract] [Full Text] [PDF]

New, J.H., Sugiyama, T., Zaitseva, E., and Kowalczykowski, S.C. (1998).
Rad52 protein stimulates DNA strand exchange by Rad51 and replication protein A.
Nature, 391, 407-410. PMID: 9450760. [Abstract] [Full Text] [PDF]
(see Nature News & Views: [News&Views] [N&V PDF]

Sugiyama, T., New, J.H., and Kowalczykowski, S.C. (1998).
DNA Annealing by Rad52 protein is stimulated by specific interaction with the complex of replication protein A and single-stranded DNA.
Proc. Natl. Acad. Sci. USA,
6049-6054. PMID: 9600915; PMCID: PMC27583. [Abstract] [Full Text] [PDF]

Solinger, J.A., Lutz, G., Sugiyama, T., Kowalczykowski, S.C., and  Heyer, W.-D. (2001).
Rad54 protein stimulates heteroduplex DNA formation in the synaptic phase via specific interactions with the presynaptic Rad51 nucleoprotein filament.
J. Mol. Biol.,
, 1207-1221. PMID: 11292336. [Abstract] [Full Text] [PDF]

Sugiyama, T. and Kowalczykowski, S.C.  (2002).
Rad52 protein associates with RPA-ssDNA to accelerate Rad51-mediated displacement of RPA and presynaptic complex formation.
J. Biol. Chem.
J. Biol. Chem. 277, 31663-31672  (published June 19, 2002 as 10.1074/jbc.M203494200). PMID: 12077133. [Abstract] [Full Text] [PDF]

Kantake, N., Madiraju, M.V.V.M., Sugiyama, T., and Kowalczykowski, S.C. (2002)
Escherichia coli RecO protein anneals ssDNA complexed with its cognate ssDNA-binding protein: A common step in genetic recombination.
Proc. Natl. Acad. Sci. USA, 99
, 15327-15332 (published November 18, 2002, as 10.1073/pnas.252633399). PMID: 12438681; PMCID: PMC137716.
[Abstract] [Full Text] [PDF]
(Recommended paper by Faculty of 1000 [Full Text])
Access the recommendation on F1000Prime

Kantake, N., Sugiyama, T., Kolodner, R.D., and Kowalczykowski, S.C.  (2003).
The recombination-deficient mutant RPA (rfa1-t11) is displaced slowly from single-stranded DNA by Rad51 protein.
J. Biol. Chem.,
278, 23410-23417 (published April 14, 2003 as doi:10.1074/jbc.M302995200). PMID: 12697761.  [Abstract] [Accepted Manuscript] [Full Text] [PDF]

Wu, Y., Sugiyama, T., and Kowalczykowski, S.C. (2006).
DNA annealing mediated by Rad52 and Rad59 proteins.
J. Biol. Chem., 281
, 15441-15449 (published online March 25, 2006 as doi:10.1074/jbc.M601827200). PMID: 16565518.
[Abstract] [Full Text] [PDF]

Wu, Y., Siino, J.S., Sugiyama, T., and Kowalczykowski, S.C. (2006).
The DNA binding preference of Rad52 and Rad59 proteins: Implications for their function in homologous recombination.
J. Biol. Chem., 281,
40001-40009. (published online October 12, 2006 as doi:10.1074/jbc.M608071200). PMID: 17040915 
[Abstract] [Full Text] [PDF]

Sugiyama, T., Kantake, N., Wu, Y., and Kowalczykowski, S.C. (2006)
Rad52-mediated DNA annealing after Rad51-mediated DNA strand exchange promotes second ssDNA capture.
EMBO J., 25,
5539-48. (published online November 9, 2006 as doi:10.1038/sj.emboj.7601412). PMID: 17093500; PMCID: PMC1679760. [Abstract] [Full Text] [PDF] [Supplementary Figure S1] [Supplementary Figure S2]

Wu, Y., Kantake, N., Sugiyama, T. and Kowalczykowski, S.C.(2008).
Rad51 Protein Controls Rad52-Mediated DNA Annealing.
J. Biol. Chem
., 283, 14883-92 (published online March 12, 2008 as doi:10.1074/jbc.M801097200). PMID: 18337252; PMCID: PMC2386943 [Abstract] [Full Text] [PDF] 

Sugiyama, T. and Kantake, N. (2009).
Dynamic regulatory interactions of Rad51, Rad52, and replication protein-A in recombination intermediates.
J. Mol. Bol. 390
, 45-55. PMID: 19445949.