Siino, Joseph




Joseph S. Siino, Jr.

B.S., 1990, Genetics - University of California, Davis

M.S., 1992, Biochemistry & Biophysics - Washington State University, Pullman, WA

Ph.D., 2002, Biochemistry & Molecular Biology - University of California, Davis

Postdoc., 2003-2007, Biochemistry, Molecular Biology & Structural Biology, University of California, Davis

2003 - 2007, American Cancer Society Postdoctoral Fellow

  Current address:

Senior Scientist
Bio-Rad Laboratories
2000 Alfred Nobel Drive
Hercules, CA 94547
Tel: (510) 232-7000

Schroth, G.P., Siino, J.S., Cooney, C.A., Th'ng, J.P., Ho, P.S., and Bradbury, E.M. (1992).
Intrinsically bent DNA flanks both sides of an RNA polymerase I transcription start site (both regions display novel electrophoretic mobility).
J. Biol. Chem., 267,

Siino, J.S., Nissen, M.S., and Reeves, R. (1995).
Replacement of conserved threonines by alanine residues in high mobility group protein HMG-I(Y): Effect on DNA binding affinity.
Biochem. Biophys. Res. Comm., 207,

Siino, J.S., Nazerov, I., Zalenskaya, I.A., Yau, P., Bradbury, E.M., and Tomilin, N.V. (2002).
Efficient end-joining of reconstituted chromatin in vitro by soluble nuclear proteins from human cells.
FEBS Letters, 527,

Zhang, K., Williams, K.E., Huang, L., Yau, P., Siino, J.S., Bradbury, E.M., Jones, P.R., Minch, M.J., and Burlingame, A.L. (2002).
Histone acetylation and deacetylation: Identification of acetylation and methylation sites of HeLa histone H4 by mass spectrometry.
Molecular and Cellular Proteomics 1,

Siino, J.S., Nazarov, I.B., Svetlova, M.P., Solovjeva, L.V., Adamson, R., Zalenskaya, I.A., Yau, P.M., Tomilin, N.V., and Bradbury, E.M. (2002).
Photobleaching of GFP labeled H2AX in chromatin: H2AX has low diffusional mobility in the nucleus.

Biochem. Biophys. Res. Comm., 297,

Zalensky, A.O., Siino, J.S., Gineitis, A.A., Zalenskaya, I.A., Tomilin, N.V., Yau, P., and Bradbury, E.M. (2002).
Human sperm/testis specific histone H2B (TSH2B).  Molecular cloning and characterization.
J. Biol. Chem., 277,

Siino, J.S., Yau, P.M., Imai, B.S., Gatewood, J.M., and Bradbury, E.M. (2003).
Effect of DNA length and histone acetylation on the thermal stability of reconstituted nucleosome particles.
Biochem. Biophys. Res. Comm., 302,

Siino*, J., Churikov*, D., Svetlova, M., Zhang, K., Gineitis, A., Bradbury, E.M., and Zalensky, A. (2004).
Novel human testis-specific histone H2B encoded by the interrupted gene on the X chromosome.
Genomics, 84,
745-756.  *Co-first authors

Zhang, K., Siino, J.S., Jones, P.R., Yau, P.M., and Bradbury, E.M. (2004).
A mass spectrometric "Western blot" to evaluate the correlations between histone methylation and histone acetylation.
Proteomics, 4,

Nikiforov, A., Svetlova, M., Solovjeva, L., Sasina, L., Siino, J., Nazarov, I., Bradbury, M., Tomilin, N. (2004). 
DNA damage-induced accumulation of Rad18 protein at stalled replication forks in mammalian cells involves upstream protein phosphorylation.
Biochem. Biophys. Res. Comm., 323,

Li, A., Maffey, A., Abbott, W.D., Conde e Silva, N., Prunell, A., Siino, J.S., Churikov, D., Zalensky, A.O., and Ausió, J. (2005).
Characterization of nucleosomes consisting of the human testis/sperm-specific histone H2B variant (hTSH2B).
Biochemistry, 44,

Wu, Y., Siino, J.S., Sugiyama, T., and Kowalczykowski, S.C. (2006).
The DNA binding preference of Rad52 and Rad59 proteins: Implications for their function in homologous recombination.
J. Biol. Chem., 281,
40001-40009. (published online October 12, 2006 as doi:10.1074/jbc.M608071200). PMID: 17040915 
[Abstract] [Full Text] [PDF]

Nimonkar, A.V., Dombrowski, C.C., Siino, J. S., Stasiak, A. Z., Stasiak, A. and Kowalczykowski, S.C. (2012). Saccharomyces cerevisiae Dmc1 and Rad51 proteins preferentially function with Tid1 and Rad54 proteins, respectively, to promote DNA strand invasion during genetic recombination.
J. Biol. Chem., 287,
28727-28737 (published online published 29 June 2012 as 10.1074/jbc.M112.373290). PMID: 22761450; PMCID: PMC3436576 [Abstract] [Full Text] [PDF]