Bianco, Piero



Piero Bianco.jpg (93131 bytes) Piero R. Bianco

B.S., Biology, 1987 - Abilene Christian University

Ph. D., Biochemistry and Molecular Biology, 1993 - University of Texas Medical School at Houston

Postdoctoral Scholar, 1994-2000 - University of California, Davis, CA

  Current address:

Associate Professor
Department of Microbiology and Immunology
Center for Single Molecule Biophysics
321 Cary Hall
University at Buffalo
The State University of New York
Buffalo, NY 14214
Office Tel: (716) 829-2599
Fax (716) 829-2158

Bianco, PR and Weinstock, GM (1994).
Automated determination of β-galactosidase specific activity.
Biotechniques 17:974-80. PMID: 7840979.

Bianco, PR and Weinstock, GM (1996).
Interaction of the RecA protein of Escherichia coli with single-stranded oligodeoxyribonucleotides.
Nucleic Acids Res. 24
:4933-9. PMID: 9016663.

Bianco, P.R. and Kowalczykowski, S.C. (1997).
The recombination hotspot Chi is recognized by the translocating RecBCD enzyme as the single strand of DNA containing the sequence 5'-GCTGGTGG-3'.
Proc. Natl. Acad. Sci. USA, 94, 6706-6711. PMID: 9192629; PMCID: PMC21222. [Abstract] [Full Text] [PDF]

Bianco, PR and Weinstock, GM (1998).
Characterization of RecA1332 in vivo and in vitro. A role for α-helix E as a liaison between the subunit-subunit interface and the DNA and ATP binding domains of RecA protein.
Genes to Cells 3
:79-97. PMID: 9605403.

Bianco, PR and Weinstock, GM (1998).
UTP is a cofactor for the DNA strand exchange reaction performed by the RecA protein of Escherichia coli.
Biochemistry 37
:7313-20. PMID: 9585545.

Arnold, D.A., Bianco, P.B., and Kowalczykowski, S.C. (1998).
The Reduced Levels of χ Recognition Exhibited by the RecBC1004D Enzyme Reflect Its Recombination Defect in Vivo.
J. Biol. Chem.,
273, 16476-16486. PMID: 9632715. [Abstract] [Full Text] [PDF]

Bianco, P.R., Tracy, R.B., and Kowalczykowski, S.C. (1998).
DNA strand exchange proteins: A biochemical and physical comparison.
Front. Biosci., 3, D570-D603. PMID: 9632377. [Abstract] [Full Text] [PDF]

Bianco, P.R. and Kowalczykowski, S.C. (1999).
RecA protein.
In: Encyclopedia of Life Sciences, Nature Publishing Group, London,   [Abstract] [Full Text] [PDF] [Preprint.PDF]

Bianco, P.R. and Kowalczykowski, S.C.  (2000).
The translocation step size and mechanism of the RecBC helicase.
Nature, 405, 368-372. PMID: 10830968.
[Abstract] [Full Text] [PDF]
(see Dispatch in Current Biology: [Abstract] [Full Text] [PDF])
(see News & Information at UC Davis News Service  [Full Text] [Printable Version])
(see Science Blog [PDF])

Bianco, P.R., Brewer, L.R., Corzett, M., Balhorn, R., Yeh, Y., Kowalczykowski, S.C., and Baskin, R.J. (2001).
Processive translocation and DNA unwinding by individual RecBCD enzyme molecules.
Nature, 409, 374-378. PMID: 11201750. [Abstract] [Full Text] [PDF]

Movies!   37°C:  1 mM ATP        23°C:   1 mM ATP;    250 µM ATP #1;    250 µM ATP #2;    no ATP
(see coverage in Biophotonics (2001) 8, 48-49   [TOC] [PDF]
(see UC Davis Division of Biological Sciences Newsletter [PDF])

Press coverage:
Capitol Public Radio - January 17, 2001 - Full transcript
San Francisco Chronicle - January 20, 2001 - excerpt
Full article - "Watch DNA Unzip in Micromovie-Molecular action film highlights potential of nanotechnology"

San Francisco Chronicle - January 24, 2001 - excerpt
Full article: "A Little Spark Of Inspiration"

San Francisco Chronicle - January 26, 2001 - excerpt

Full article: "Never Metatag We Didn't Like"

News & Information at UC Davis News Service  [Full Text]
Nanotech Dreams - The Scientist 16[5]:34, Mar. 4, 2002 - Excerpt Full article
Davis  Community  Television
- February 8, 2001 7:00 PM  - "Get The Word Out." Double helix unwinds on camera. Stephen Kowalczykowski interviewed by Jon Price. (Rerun on Tues Feb 13, 2001 at 7:30 PM). [VIDEO]

Spies, M., Bianco, P.R., Dillingham, M.S., Handa, N., Baskin, R.J., and Kowalczykowski, S.C.  (2003)
A molecular throttle: The recombination hotspot
c controls DNA translocation by the RecBCD helicase.
, 114, 647-654. PMID: 13678587.
[Abstract] [Full Text] [PDF] [Featured Article] [Supplemental Table S1] [Supplemental Table S2] [Supplemental Table S3] [Supplemental Movie S1] [Supplemental Movie S2] [Supplemental Movie S3]
News & Features at  [Full Text] [PDF])
News & Information at UC Davis News Service  [Full Text] [Printable Version])
News & Information at UC Davis News Service  [Full Text] [Printable Version])

Handa, N., Bianco, P.R., Baskin, R.J., and Kowalczykowski, S.C. (2005)
Direct visualization of RecBCD movement reveals co-translocation of the RecD motor after
Mol. Cell,
17, 745-750. PMID: 15749023. [Abstract] [Full Text] [PDF] [Supplemental Data] [Movie 1 - Chi0] [Movie 2 - Chi+]
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Bianco, P.R. and Kowalczykowski, S.C. (2005).
RecA protein.
: “Encyclopedia of Life Sciences”, John Wiley & Sons, Ltd: Chichester
,  [doi:10.1038/npg.els.0003925] [Abstract] [PDF] [HTML]