– Lab jobs
– Yeast protocols
- Basics on Saccharomyces cerevisiae
- General tips
- DNA extraction using CTAB
- DNA extraction using PCIA
- DNA extraction in agarose plugs (for Pulse-Field gel)
- DNA extraction using KAc and Isopropanol
- Gene expression induction with Galactose (like the HO endonuclease with the GAL-HO construct)
- Immunoprecipitation
- Irradiation (gamma-ray)
- Media recipes
- MMS handling
- PCR on colony (fast transformant screening)
- Plasmid transfer from E. coli to S. cerevisiae
- Proteins extraction using TCA
- Psoralen crosslinking and DNA extraction
- Psoralen crosslinking (variation very severe crosslinking density)
- Psoralen de-crosslinking
- Yeast restric
- Yeast sporulation
- Yeast spotting assay
- Yeast strains archiving
- Yeast transformation / Fast yeast transformation
– Biochemistry protocols
- DNA strand exchange reaction (D-loop assay)
- ATPase assay
- Nucleoprotein gel assay for Rad51-DNA complexes
- Fermentor protocol
– Molecular biology various
- Ligation (sticky ends)
- Ligation (blunt ends)
- DNA precipitation with Ethanol and salts
- Dialysis tube preparation
- Dephosphorylation of plasmid
- Denaturing purification of insoluble proteins
- Lab Solutions
– Gels
- Antibody production
- Western blot
- Southern blot
- Northern blot
- 2D-gel
- Protein polyacrylamide gel
- DNA polyacrylamide gel
- Pulse-Field gel
- PCR
- Real Time qPCR
– E. coli