Kowalczykowski Lab Home Page

 

Stephen Kowalczykowski, Professor
University of California, Davis
Sections of Microbiology and of Molecular and Cellular Biology

Biochemistry and Molecular Biology, Biophysics, Genetics, and Microbiology Graduate Groups
Briggs Hall - Rm. 310
One Shields Ave
Davis, CA 95616-8665

E-MAIL:   sckowalczykowski@ucdavis.edu
PHONE (office):  53
0-752-5938      FAX (office):  530-752-5939

   PHONE (department):  530-752-0260   FAX (department):  530-752-9014
Focus in Chromosome Dynamics at U.C. Davis

  Research Interests Publications People

Recent papers and papers in press

Our research interests include:

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Molecular mechanism of genetic recombination
DNA strand exchange proteins
Homologous pairing

Recombination-intermediate structures
DNA helicases
DNA processing
Recombination hotspots

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Biochemistry of DNA helicases
DNA unwinding and ATP hydrolysis
Translocation along DNA
Molecular motor mechanisms
Reconstituted in vitro reactions

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Protein-nucleic acid interactions
Atomic force (scanning probe) microscopy
Single-molecule visualization
Equilibrium and kinetic properties
Fluorescence-based assays

 

[Click here to see a movie of translocation/unwinding by RecBCD helicase along DNA]

  laser optical trap
Streptavidin-coated bead (1m)

More movies: 
1 mM ATP
250 µM ATP #1
250 µM ATP #2
no ATP

News Flashes!

159. Handa, N. and Kowalczykowski, S.C. (2007).  A RecA mutant, RecA730, suppresses the recombination deficiency of the RecBC1004D–c* interaction in vitro and in vivo. J. Mol. Biol., 365, 1314-1325 (published online November 1, 2006 as doi:10.1016/j.jmb.2006.10.090).
[Abstract]  [Full Text]  [PDF]

 

160. Galletto, R. and Kowalczykowski, S.C. (2007). RecA. Curr. Biol. 17, R395-R397.
[Full Text] [PDF]



161. Nimonkar, A.V., Amitani, I., Baskin, R.J., and Kowalczykowski, S.C. (2007).  Single-molecule imaging of Tid1/Rdh54, a Rad54 homolog that translocates on duplex DNA and can disrupt joint molecules. J. Biol. Chem., 282, 30776 - 30784 (published online August 16, 2007 as doi:10.1074/jbc.M704767200).
[Abstract]  [Full Text]  [PDF] [Supplementary Information]

 

162. Spies, M., Amitani, I., Baskin, R.J., and Kowalczykowski, S.C. (2007). RecBCD enzyme switches lead motor subunits in response to c-recognition. Cell, 131, 16, 694-705  (doi:10.1016/j.cell.2007.9.023).
[Abstract]  [Full Text]  [PDF]
 [Supplementary Information]
(see Cell [PDF])

 

163. Wu, Y., Kantake, N., Sugiyama, T., and Kowalczykowski, S.C. (2008).  Rad51 protein controls Rad52-mediated DNA annealing.  In press. (published online March 12, 2008 as doi:10.1074/jbc.M801097200).
[Abstract] [PDF]

 

 

NIH Videocast: DNA Repair Interest Group
Biochemistry of Recombinational DNA Repair: Common Themes
Author/Sponsor: Dr. Steve Kowalczykowski, UC Davis
Tuesday, January 20, 2004; Total Running Time: 01:22:40 (Requires RealPlayer software )
Slide Show  or  PDF Presentation

Highly cited paper selected by ISI Essential Science Indicators: "Biochemistry of homologous recombination in Escherichia coli," (Microbiol. Rev. 58 [3]: 401-65, September 1994). This paper ranks among the top 15 highly cited papers in the field of Microbiology, with a total of 511 citations to date.
(in-cites, April 2002 - Citing URL: http://www.in-cites.com/BI-april2002.html )         
Read interview with Dr. Stephen Kowalczykowski

Nanotech Dreams - The Scientist 16[5]:34, Mar. 4, 2002 - excerpt
Full article

Davis  Community Television - February 8, 2001 7:00 PM: Get The Word Out - "Double helix unwinds on camera." Stephen Kowalczykowski interviewed by Jon Price.
(Rerun on Tues Feb 13, 2001 at 7:30 PM). [VIDEO]

   

Scott, Eugenie C., ... your name et al. 2004. The Morphology of Steve. Annals of Improbable Research, July-slash-August. pp. 24-29.pdf
[PDF]