Review Articles



Forget, A.L., Dombrowski, C.C., Amitani, I., and Kowalczykowski, S.C. (2013)
Exploring protein-DNA interactions in 3D using in situ construction, manipulation, and visualization of individual DNA dumbbells with optical traps, microfluidics, and fluorescence microscopy.
Nature Protocols, 8
, 525-538. 
PMID: 23411634 [Abstract] [Full Text] [PDF]

Amitani, I., Liu, B., Dombrowski, C.C., Baskin, R.J., and Kowalczykowski, S.C. (2010)
Watching individual proteins acting on single molecules of DNA.
Single Molecule Tools: Fluorescence Based Approaches, Part A, Methods Enzymol., 472, Nils G. Walters, editor, Burlington: Academic Press, 261-291
ISBN: 978-0-12-374954-3; doi: 10.1016/S0076-6879(10)72007-3PMID: 20580968; PMCID: PMC3119597  [Abstract] [Full Text] [PDF]

Hilario, J. and Kowalczykowski, S.C. (2010)
Visualizing protein-DNA interactions at the single-molecule level.

Curr. Opin. Chem. Biol.,
14, 15-22. PMID: 19945909; PMCID: PMC2819561 [Abstract] [Full Text] [PDF]

Forget. A.L. and Kowalczykowski, S.C. (2010)
Single-molecule imaging brings Rad51 nucleoprotein filaments into focus.
Trends in Cell Biology,
20, 269-276 (published online March 17, 2010, as doi:10.1016/j.tcb.2010.02.004). PMID: 20299221;
PMCID: PMC2862779 [Abstract] [Full Text] [PDF]

Nimonkar, A.V. and Kowalczykowski, S.C. (2009)
Second-end DNA capture in double-strand break repair: How to catch a DNA by its tail.

Cell Cycle, 8
, 1816-1817. PMID: 19471119; PMCID: PMC3051008. [Full Text]  [PDF]

Carreira, A. and Kowalczykowski, S.C. (2009)
BRCA2: Shining light on the regulation of DNA-binding selectivity by RAD51.
Cell Cycle,
21, 11-13. PMID: 19838057; PMCID: PMC3050000. [Full Text]  [PDF]

Kowalczykowski, S.C. (2008)
Structural biology: Snapshots of DNA repair.
Nature, 453, 463-466 (published online May 21, 2008 as doi:10.1038/453463a). PMID: 18497811. [Full Text] [PDF]

Dillingham, M.S. and Kowalczykowski, S.C. (2008)
RecBCD enzyme and the repair of double-stranded DNA breaks.
Microbiol. Mol. Biol. Rev., 72,
642–671 (published online as doi:10.1128/MMBR.00020-08). PMID: 19052323; PMCID: PMC2593567 [Abstract] [Full Text] [PDF]

Galletto, R. and Kowalczykowski, S.C. (2007)
Curr. Biol, 17
, R395-R397. PMID: 17550759.
[Full Text] [PDF]

Linder, P. and Kowalczykowski, S.C. (2006)
Helicases and NTP-driven nucleic acid machines: Structure, function and roles in human disease.
Nucl. Acids Res., 34,
4081. PMCID: PMC3303210. [Full Text] [PDF]

Bianco, P.R. and Kowalczykowski, S.C. (2005)
RecA protein.
: “Encyclopedia of Life Sciences”, John Wiley & Sons, Ltd: Chichester
,  [doi:10.1038/npg.els.0003925]
)  [Abstract] [PDF] [HTML]

Kowalczykowski, S.C. (2005)
Catalyst of a catalyst.
Nature, 433
, 591-592. PMID: 15703734 
[Full Text] [PDF]

Spies, M., Dillingham, M.S., and Kowalczykowski, S.C. (2005)
DNA helicases.
“McGraw-Hill Encyclopedia of Science & Technology”, New York, N. Y.

Spies, M. and Kowalczykowski, S.C. (2005)
Homologous recombination by the RecBCD and RecF pathways.
 “The Bacterial Chromosome”, N.P. Higgins, ed., (ASM Press, Washington, D.C.), 389-403.
[PDF] [Preprint]

Spies, M., Dillingham, M.S., and Kowalczykowski, S.C. (2003)
DNA helicases.
McGraw-Hill Yearbook of Science & Technology”, New York, N. Y., 95-98 [PDF]
[scanned-PDF]  [Page proof]

Chédin, F. and Kowalczykowski, S.C.  (2002)
A novel family of regulated helicases/nucleases from Gram-positive bacteria: insights into the initiation of DNA recombination.
Mol. Microbiol.  43
, 823-834. PMID: 11929535.
[Full Text] [PDF]

Kowalczykowski, S.C. (2002)
Molecular mimicry connects BRCA2 to Rad51 and recombinational DNA repair.
Nature Struct. Biol., 9,
897-899. PMID: 12447354.
[Full Text] [PDF]

Seitz, E.M., Haseltine, C. A., and Kowalczykowski, S.C.  (2001)
DNA Recombination and repair in the Archaea.
”Archaea:  Ancient Microbes, Extreme Environments, and the Origin of Life”, P. Blum, ed.,
Advances in Applied Microbiology, 50
[PDF] [Preprint]

Dillingham, M. S. and Kowalczykowski, S.C.  (2001)
A Step Backward in Advancing DNA Replication: Rescue of Stalled Replication Forks by RecG. 
Mol. Cell, 8,
734-736. PMID: 11684009. [Abstract] [Full Text] [PDF]

Kowalczykowski, S.C.  (2000)
Some assembly required.
Nature Structural Biology, 7,
1087-1089. PMID: 11101884.
[Full Text] [PDF] [Extra Figure]

Kowalczykowski, S.C. (2000)
Initiation of genetic recombination and recombination-dependent replication.

Trends Biochem. Sci., 25
, 156-165. PMID: 10754547. [Abstract] [Full Text] [PDF]

Kowalczykowski, S.C. and von Hippel, P.H. (eds.) (2000)
The recombination–replication interface
Trends Biochem. Sci., 25.  [Full Text] [Contents] [Summary and Table of Proteins]

Harmon, F.G. and Kowalczykowski, S.C. (2000) 
Coupling of DNA helicase function to DNA strand exchange.
Methods Mol. Biol., 152
, 75-89. 
[Abstract] [Full Text] [PDF]

Bianco, P.R. and Kowalczykowski, S.C. (1999)
RecA protein.
In: Encyclopedia of Life Sciences, Nature Publishing Group, London,   [Abstract]
 [Full Text] [PDF] [Preprint.PDF]

Arnold, D.A. and Kowalczykowski, S.C. (1999)
RecBCD helicase/nuclease.
In: Encyclopedia of Life Sciences, Nature Publishing Group, London,   [Abstract] [Full text] [PDF] [Preprint.PDF]

Bianco, P.R., Tracy, R.B., and Kowalczykowski, S.C. (1998)
DNA strand exchange proteins: A biochemical and physical comparison.
Front. Biosci., 3, D570-D603. PMID: 9632377. [Abstract] [Full Text] [PDF]

Mazin, A.V. and Kowalczykowski, S.C. (1998)
The synergistic interaction between RecA protein and SSB protein during DNA strand exchange.
In: "Modern Concepts in Evolutionary Genetics", (V.K. Shumny and A.L. Markel, eds.), Nauka, Novosibirsk, Russia, Proceedings of the Conference. [Preprint.PDF]

Kowalczykowski, S.C. and Zarling, D. (1995)
Homologous recombination proteins and their potential applications in gene targeting technology.
In: "Gene Targeting", (M.A. Vega, ed.) CRC Press, Inc., Chapter 7, 167-210. [PDF]

Kowalczykowski, S.C., Dixon, D.A., Eggleston, A.K., Lauder, S.D., and Rehrauer, W.M. (1994)
Biochemistry of homologous recombination in Escherichia coli.
Microbiol. Rev., 58, 401-465. PMID: 7968921; PMCID: PMC372975. [Abstract] [Full Text] [PDF]
(Highly cited paper selected by ISI Essential Science IndicatorsThis paper ranks among the top 15 highly cited papers in the field of Microbiology, with a total of 511 citations to date.) (in-cites, April 2002 - Citing URL:
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Kowalczykowski, S.C. and Eggleston, A.K. (1994)
Homologous pairing and DNA strand exchange proteins.
Annu. Rev. Biochem., 63, 991-1043. PMID: 7979259. [Full Text] [PDF]

Kowalczykowski, S.C. (1994)
In vitro reconstitution of homologous recombination reactions.
Experientia, 50, 204-215. PMID: 8143794. [Abstract] [Full Text] [PDF]

Kowalczykowski, S.C. (1991)
Biochemistry of genetic recombination: Energetics and mechanism of DNA strand exchange.
Annu. Rev. Biophys. Biophys. Chem. 20, 539-575. PMID: 1831022.

Eggleston, A.K. and Kowalczykowski, S.C. (1991)
An overview of homologous pairing and DNA strand exchange proteins.
Biochimie 73, 163-176. PMID: 1653031. [Abstract] [Full Text] [PDF]

Kowalczykowski, S.C. (1991)
Biochemical and biological function of Escherichia coli RecA protein: behavior of mutant RecA proteins.
Biochimie 73, 289-304. PMID: 1883888. [Abstract] [Full Text]

Kowalczykowski, S.C. and Roman, L.J. (1990)
Reconstitution of homologous pairing activity in vitro dependent upon the combined activities of purified E. coli RecA, RecBCD, and SSB proteins.
In: Molecular Mechanisms in DNA Replication and Recombination, C. Richardson and R. Lehman, eds. (Alan R. Liss, Inc. New York), (New Series) 127, 357-373. [PDF]

Kowalczykowski, S.C.  (1990)
Thermodynamic data for protein-nucleic acid interactions.
In: Landolt-Bornstein: Numerical Data and Functional Relationships in Science and Technology (New Series) Group VII: Biophysics, Nucleic Acids 1d, W. Saenger, ed. (Berlin: Springer-Verlag), 244-263. [PDF]

Kowalczykowski, S.C. (1987)
Mechanistic aspects of the DNA strand exchange activity of Escherichia coli recA protein.
Trends Biochem. Sci. 12, 141-145. [Abstract] [PDF]

von Hippel, P.H., Kowalczykowski, S.C., Lonberg, N., Newport, J.W., Paul, L.S., Stormo, G.D., and Gold, L. (1983)
Autoregulation of expression of gene 32 protein of bacteriophage T4: A quantitative analysis.
In: The Bacteriophage T4, C.K. Matthews, E.M. Kutter, G. Mosig and P.B. Berget, eds. (Washington, D.C.: American Society for Microbiology), pp. 202-207.  [PDF]

Kowalczykowski, S.C., Bear, D.G., and von Hippel, P.H. (1981).
Single-stranded DNA binding proteins.
In: The Enzymes, P.D. Boyer, eds. (New York: Academic Press), pp. 373-442. [PDF]